途游斗地主

EN

XUANZEHESHIDEXIBAOMOXING,NIDEJIBINGYANJIUHEYAOWUSHAIXUANJIUCHENGGONGLEYIBAN

「ZHONGLIXUNTAQIANBAIDU」,YONGLAIXINGRONGYAOWUSHAIXUAN,ZAIHESHIBUGUO。BAIYUNIANQIAN,XIBAOZUOWEIMOXINGXITONGBEIYONGYUYAOWUSHAIXUANHEJIBINGYANJIU,RANGZHEIYIFANZA、KUNNANDEGONGZUOZHUBUMAIXIANG「LIUANHUAMING」,BANGZHUYANJIURENYUANSHAIXUANCHULEBUSHAOGENGWEIANQUAN、YOUXIAODEYAOWU。JINTIAN,JIURANGWOMENYIQIZOUJINJIZHONGDAIBIAOXINGDEXIBAOMOXING。

原代细胞

YUANDAIXIBAOSHITONGGUOMEIJIEHUOWULIFANGFACONGHUOTIZUZHI(LIRUHUOJIANCAILIAO)HUOZHEXUEYEZHONGZHIJIEFENLIHUOQU,BINGZAITIWAIPEIYANGDEXIBAO。YOUYUYUANDAIXIBAOJINGLIJISHAODEQUNTIBEIZENG,BAOLIULEQIYUANSHIZUZHIDEBIAOXINGHEJIYINXINGTEZHENG,YINCIBILIANXU(ZHONGLIUHUORENGONGYONGSHENGHUADE)XIBAOXIGENGNENGDAIBIAOQILAIYUANZUZHIDEZHUYAOGONGNENGCHENGFEN。

JINGUANYOUDIANZHONGDUO,DANBUKEFOURENDESHI,YUANDAIXIBAODEFENLIKENENGFEISHI、FEILI,ERQIEMEIZHONGXIBAOLEIXINGDOUYOUQIDUTEDEYINGYANGXUQIU。TONGSHI,XIBAOCHANLIANGZAIPEIYANGGUOCHENGZHONGHAIKENENGFASHENGBIANHUA,SUIZHESHIJIANDETUIYI,XIBAOZAIPEIYANGHESHUAILAODEGUOCHENGZHONGHUIBIANDEBUYIKUOZENG。

途游斗地主RENTIDEYUANDAIXIBAOKEYONGYUQUEDINGCONGDONGWUMOXINGWAITUISUODERENLEISHUJUDEZHUNQUEXING,RENGANXIBAOBIANSHIYIXIANGYIZAITIWAIYAOWUDULIXUELINGYUGUANGFANSHIYONGDEYUANDAIXIBAOSHILI。

图 1  在原代肝细胞中进行药物/化合物的吸收、分配、代谢、排除以及毒性(ADME/TOX)的研究,对于评估药物安全及药代动力学至关重要。

JINGUANYUANDAIGANXIBAOZAIDULIXUEYANJIUZHONGDEJIAZHIYIJINGDEDAOLEGUANGFANDERENKE,DANYOUYUGONGTIBUZU、CHENGBENJIAOGAO、BUTONGXIBAOLAIYUANJIANJIAODADEYICHUANCHAYI、GANXIBAOZAITIWAIDEZENGZHINENGLIYOUXIAN,YIJIYUANDAIXIBAOWUFABIMIANDEQUEDIAN(SHOUMINGDUAN、TINEIGONGNENGDEXUNSUSANGSHI),XIANZHILEYUANDAIXIBAOZAIDULIXUEYANJIUZHONGDEYINGYONG。

途游斗地主ZAIZHEIZHONGQINGKUANGXIA,XIBAOXIYINGYUNERSHENG。

细胞系

XIBAOXIZHIDESHIYUANDAIXIBAOPEIYANGWUJINGSHOUCICHUANDAICHENGGONGHOUSUOFANZHIDEXIBAOQUNTI,ZAIQICUNHUOQIJIAN,JUYOUZUIGAOSHENGZHANGNENGLIDEXIBAOJIANGZHANJUZHUDAODIWEI,DAOZHIXIBAOQUNTIZAIJIYINXINGHEBIAOXINGSHANGKEYIDADAOYIDINGCHENGDUDEJUNYIXING,GUERKEYIYONGYUJIEJUEYUANDAIXIBAOPEIYANGZHONGSHOUMINGDUANHEKUOZENGSHOUXIANDEWENTI。

XIANGBIYUYUANDAIPEIYANGDEXIBAO,XIBAOXIGENGWEIJUNYIHEBIAOZHUNHUA,ZAIYAOWUSHAIXUANHEDULIXUEYANJIUZHONGJUYOUZHONGYAOZUOYONG,JUGELIZI:

途游斗地主HepG2 XIBAOXI(YUANZIGANAIZUZHI)SHIZHENGCHANGGANXIBAODEYOUXIAOTIDAIWU,HepG2 XIBAOXIXIANSHICHUYUTIANRANGANXIBAOXIANGSIDEXINGTAIHEGONGNENGTEXING,KEYIHECHENGGANXIBAOXIANGGUANDEXUEQINGDANBAI(QINGDANBAI、α2-JUQIUDANBAI、SIANSUANDANBAIMEIYIZHIJI A1、α-KANGYIDANBAIMEI,ZHUANTIEDANBAIHEXUEXIANWEIDANBAIRONGMEIYUANDENG)。

途游斗地主TU 2  CHANGYONGXIBAOXISHIYITU

途游斗地主XIBAOXIKEYIFENWEIYOUXIANXIBAOXIHEWUXIANXIBAOXI,YOUXIANXIBAOXISHOUMINGYOUXIAN,ZAISHUAILAOZHIQIANKECHUANDAI 20-80 CI;WUXIANXIBAOXIZEJUYOUWUXIANFANZHIDENENGLI,JIYONGSHENGHUA。YUANZIGONGJINGAIXIBAODE HeLa XIBAOXISHIZUIZHUMINGDEXIBAOXI,YESHIYIXUEYANJIUZHONGDEZHONGYAOGONGJU。

途游斗地主TONGYANGDE,XIBAOXIDEQUEDIANYEHENMINGXIAN:

途游斗地主1. RONGYIFASHENGZIRANHUOYOUDAOTUBIAN,ZAIJINGLIFEICHANGDUODECHUANDAIHOU,XIBAOXIKENENGHUIFASHENGYIXIEJIYINXINGNAIZHIBIAOXINGDEGAIBIAN;

途游斗地主2. XIBAOWURAN,QUANSHIJIESHIYONGDEXIBAOXIZHONGYOUYUE 15–20% BEICUOWUSHIBIEHUOBEIQITALEIXINGDEXIBAOWURAN。

JINGUANRUCI,XIBAOXIZUOWEISHIYIZHONGJIANDANERYOUXIAODEGONGJU,RENGSHIXUESHULINGYUHEZHIYAOXINGYEYANJIUDEZHUYAOSHOUDUAN,BUDEBUSHUO,YONGSHENGAIXIBAOXISHIYANJIUAIZHENGSHENGWUXUEHECESHIKANGAILIAOFADEBAOGUIMOXING。

干细胞

途游斗地主JINNIANLAI,ZAISHENGWUZHIYAOLINGYU,YANJIUDESIWEIFANGSHIZHUJIANCONGJIYUYUANDAIHEXIBAOXIDEXITONGZHUANXIANGGENGQIANGDA、GENGXIANSHIHEGENGJUGEXINGHUADEGANXIBAOXITONG。

途游斗地主GANXIBAOJUYOUWUXIANDEZENGZHINENGLIHECHANSHENGGEZHONGLEIXINGXIBAODEKESUXING,XIANGBIYUYUANDAIXIBAOHEXIBAOXI,QIZAIFAYUSHENGWUXUE、JIBINGJIANMOHEXIBAOZHILIAOLINGYUJUYOUJUDADEQIANLI。

GENJUBUTONGDEFENHUAQIANNENG,GANXIBAOKEFENWEIQUANNENGGANXIBAO、DUONENGGANXIBAO、DANNENGGANXIBAO;GENJUFASHENGXUELAIYUANJINXINGFENLEI,GANXIBAOYOUKEYIFENWEICHENGTIGANXIBAOYIJIPEITAIGANXIBAO。

成体干细胞

CHENGTIGANXIBAO,SHIZHICUNZAIYUYIZHONGYIJINGFENHUAZUZHIZHONGDEWEIFENHUAXIBAO,ZHEIZHONGXIBAONENGGOUZIWOGENGXINBINGQIENENGGOUTEHUAXINGCHENGZUCHENGGAILEIXINGZUZHIDEXIBAO。

途游斗地主GUSUIJIANCHONGZHIGANXIBAO(BM-MSCs)YIJIZAOXUEGANXIBAO(HSCs)BIANSHISHICONGCHENGRENZUZHIZHONGFENLICHULAIDE,JUYOUZIWOGENGXINDENENGLIDECHENGTIGANXIBAO,ZHEIXIEDUONENGCHENGTIGANXIBAOBEIGUANGFANYONGZUOYUCEYAOWUDUXINGDETIWAIXIBAOMOXING。

途游斗地主Bio-Techne KETIGONG BM-MSCs JI HSCs FENLI、JIANDING、PEIYANG、YOUDAOFENHUADEQUANTAOJIEJUEFANGAN,YI MSCs DEYANJIUWEILI:

胚胎和诱导性多能干细胞

PEITAIGANXIBAO(ESCs)SHIZHIYOUPEITAINEIXIBAOTUANHUOYUANSHISHENGZHIXIBAOJINGTIWAIYIZHIPEIYANGERSHAIXUANCHUDEXIBAO。1998 NIAN,YANJIURENYUANCONGPEIPAOJIEDUANDENEIXIBAOTUANZHONGFENLICHULEDIYIGERENPEITAIGANXIBAOXI。2007 NIAN,CHENGRENTIXIBAOBEIZHONGXINBIANCHENGWEIDUONENGXINGGANXIBAO,BEICHENGWEIRENLEIYOUDAODUONENGGANXIBAO(iPSCs)。

RENLEIPEITAIGANXIBAOHEYOUDAODUONENGGANXIBAOBEITONGCHENGWEIDUONENGGANXIBAO,NENGGOUZHANGQIZIWOGENGXIN,QIEKEYIXIANGSANGEPEICENG(NEIPEICENG,ZHONGPEICENGHEWAIPEICENG)DEXIBAOLEIXINGFENHUA。ZHEIXIETEXINGSHIDETAMENCHENGWEIPINGGUYAOWUHEYOUDUHUAHEWUDEYOULIGONGJU。

图 3  使用 StemXVivo Hepatocyte Differentiation Kit 将 iPSK3 人诱导多能干细胞再分化为肝细胞样细胞,并将细胞接种于 96 孔板中,使用肝毒性化合物(Carboplatin、Doxorubicin、Crizotinib、Tacrine)进行筛选。细胞活力(A)和脂质代谢(B)分析表明,肝毒性化合物对 iPSCs 衍生的肝细胞的毒性具有剂量依赖性。

ZAIWEIFENHUAZHUANGTAIXIA,DUONENGGANXIBAOHAISHIFAYUDULIXUEDEZHONGYAOTIWAIMOXING。ZHONGSUOZHOUZHI,PEITAIFAYUSHIYIGETEBIEYISHOUYAOWUHEYOUDUHUAHEWUQINHAIDESHIQI,DONGWUMOXINGHENNANGAIKUOYAOWUDUIRENLEIFAYUZAOQISHIJIANDEYINGXIANG,ERDUONENGGANXIBAOYINQITEXING,WEIYANJIUPEITAIFASHENGGUOCHENGZHONGDEYAOWUDUXINGTIGONGLEYIGEDUTEQIEKEXINGDEPINGTAI。

CIWAI,DUONENGGANXIBAOSUOJUYOUDEFENHUAWEISUOYOUXIBAOLEIXINGDENENGLI,DUIYUYANJIUXISHAOHUONANYIFENLIDEXIBAOLEIXING(LIRUSHENJINGYUANHUOXINJIXIBAO)LAISHUOJIJUJIAZHI。XIANGGUANYANJIUBIAOMING,DUONENGGANXIBAODUIJIBINGJIANMO,YAOWUSHAIXUANHEDUXINGCESHIJUYOUJIAODADEBANGZHU。

ZUIHOU,RENYOUDAODUONENGGANXIBAOHAIKEYIJIANLIJIBINGGEXINGHUADEXIBAOMOXING,YIXIEZHUTANGNIAOBING、NANGXINGXIANWEIHUA、PAJINSENSHIBINGHEJINGSHENFENLIEZHENGDENGJIBINGDEYANJIU。JUGELIZI,CONGHUANYOUJIAZUXINGZIZHUSHENJINGGONGNENGYICHANG(YIZHONGHANJIANDE、ZHIMINGDEYICHUANXINGJIBING,KEYIYINGXIANGSHENJINGJIXIBAOXIDECHANSHENG)DEHUANZHEDE iPSCs YANSHENGCHUDESHENJINGJIQIANTIXIBAO,ZAIJIANGZHEIXIEQIANTIXIBAOYONGYUXINYAOSHAIXUAN,YOUZHUYUSHEJIGENGANQUAN、GENGYOUXIAODEZHILIAOFANGAN。

 

图 4  采用 iPSCs 衍生的细胞进行个性化毒性检测

DUONENGGANXIBAODEJIANDINGYUYANJIUBAOKUOPEIYANG、FENHUA、YANZHENGDENGHUANJIE,Bio-Techne KETIGONGXIANGGUANDEPEIYANGJI、SHIJIHE、XIBAOYINZIJIXIAOFENZIHUAHEWU:

DANGRAN,BULUNSHIYUANDAIXIBAO,HAISHIXIBAOXI,YIHUOSHIGANXIBAO,ZAISHIJIYINGYONGZHONG,DOULIBUKAIJUTIDEXIBAOPEIYANG。XIBAOPEIYANGZUOWEIYIZHONGZAITIWAIMONITINEIHUANJING,SHIXIBAOSHENGCUN、SHENGZHANG、FANZHIBINGWEICHIZHUYAOJIEGOUHEGONGNENGDEYIZHONGFANGFA,YUYANJIUDECHENGGONGYUFOUXIXIXIANGGUAN。

 

以上文字和图片来源于Bio-techne

 

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